Simultaneous phycoremediation of petrochemical wastewater and lipid production by Chlorella vulgaris

Abstract A novel strategy of using microalgae Chlorella vulgaris for simultaneous bio-treatment petrochemical wastewater and lipid production was developed in the present study. Phycoremediation carried out 30 days. The profile fatty acids identified, specifications biodiesel including saponification value, iodine cetane number, long-chain saturated factor, cold filter plugging point, cloud allylic position equivalent bis-allylic were predicted by BiodieselAnalyzer® software. Besides, polycyclic aromatic hydrocarbons determined both samples produced lipid. observed data showed that from C. superior to petrodiesel terms suitability diesel engines. Moreover, contamination can influence expression a variety genes algae. To investigate effectiveness on synthesis as well three photosynthesis genes, real-time polymerase chain reaction assay used quantify transcript levels PsaB (photosystem I center protein subunit B), psbC (an integral membrane component photosystem II), rbcL (a large ribulose-1,5-bisphosphate carboxylase oxygenase). Furthermore, gene level accD (acetyl-coenzyme carboxyl transferase beta, chloroplastic) studied discover effect production. results when diluted (50%) media cultivation, these significantly increased. For 50% wastewater, maximum removal BOD, COD, total nitrogen, phosphor has been 30.36%, 10.89%, 69.89%, 92.59%, respectively.